Health Science

A Laminar Airflow Hood (LAFH)/ Laminar Aseptic Hoods, or a workbench, is a primary engineering control device which provides the following services during aseptic compounding: Clean air to the critical sites (immediate aseptic compounding area), Constant flow of air out of the work area to prevent the entry of room air, and Outward flow of

Following three culture media are commonly used to perform the tests for sterility: Nutrient Broth : This medium is used for aerobic microorganisms and have the following features: The value of redox potential (Eh) of this medium is too high to allow the growth of anaerobes. The culture medium, such as soyabean casein digest broth, Hartley’s

For the evaluation of efficiency, potency, and reactivity of disinfectants following techniques are used: Tube dilution and agar plate method, Cup-plate, filter paper, and cylinder plate method, Ditch-plate or giant colony method, Kelsey-Sykes test, In-use dilution test, Phenol coefficient test – Rideal-Walker test, and Chick-Martin test. Tube Dilution and Agar Plate Method The chemical disinfecting

Extent of antimicrobial action, rate of disinfection, and potency of a disinfectant depends on the following factors: Concentration of Disinfectant : Concentration of the disinfectant directly influences the rate of killing microorganisms. The curve obtained between the efficiency and concentration of disinfectant is generally exponential, rather than linear. Temperature : Normally, on increasing the temperature

Disinfectants are classified on the basis of the following factors: Based on Consistency : On the basis of consistency or state of matter, disinfectant are of two types: Liquid (e.g., alcohols, phenols), and Gaseous (e.g., formaldehyde vapour). Based on Spectrum of Activity : On the basis of spectrum of activity, disinfectant are of three types: High Level

Use of fertilised, embryonated chicken eggs is one of the cost-effective and convenient methods of cultivation for many animal viruses. A needle or syringe is put through a hole drilled in the shell to introduce the virus aseptically into the embryonated chicken eggs. The whole is sealed with paraffin and the eggs are incubated for 2−3 days

Industrial fermentation processes are mostly operated under vigorous aeration, maintaining the supply of sterilised air to the fermenter. Air filters should be attached to air input and exhaust ports for preventing the contamination of fermentation by air-borne microorganisms or of environment by aerosols generated within the fermenter. These air filters made up of glass fibre,

Sterile products are free of microbes, pyrogens, and particulates. They also possess high standards of purity and quality. Therefore, the sterilisation methods used during the manufacturing of sterile products should be evaluated and subjected to complex validation procedures. The final fate of sterility relies on official sterility tests. A manufacturing procedure is said to be

Salmonella typhi and Escherichia aerogen bacteria obtain carbon from citrate. This ability of utilizing citrate as the carbon and energy source is used as a distinguishing test for some Grods. Principle Simmons citrate agar containing sodium citrate as the carbon source is used to detect whether or not citrate is utilized by the organism. This

Transmission electron microscope utilise an electron beam which passes through and interacts with an ultra-thin sample to produce an image, which is then magnified and focused on an imaging device (a fluorescent screen), on a photographic film layer, or is detected with a sensor (a CCD camera). Max Kroll and Ernst Ruska designed the first