February 17, 2023

Culture is a procedure during which microorganisms are cultivated in a medium; if a culture has the population of a single type of microorganism; it is known as a pure culture. Usually, isolation and growth of pure cultures of single-celled microbes carried under aseptic conditions needs sterilised instruments in addition to filtered and still air.

To cultivate a specific microbe, following methods are used : Methods of Culturing Aerobic Bacteria : An atmosphere having oxygen in ample amount facilitates the growth of aerobic or facultative bacteria. For this, a large surface area of medium is used to increase the exposure. Numerous devices, e.g., Fernbach flask, Kolle flask, and Roux bottle