August 26, 2023

Human and other mammalian genomes are very large, therefore, vectors are needed to clone large DNA fragments. Many vectors have been prepared that aid in the characterisation and expression of large genes or gene complexes. These vectors exhibit varied cloning capacity (table). Table Maximum DNA Insert Possible with Different Cloning Vectors Vectors Hosts Insert Size

Cloning vector is a DNA molecule in which when a foreign DNA is integrated achicves the capability to replicate it within itself to produce many clones of recombinant DNA. Characteristics of a Cloning Vector Its size should be small. It should bear the capability to self-replicate within the host cell. It should carry a restriction