Insertion of Target DNA Into a Vector
The target DNA or the DNA insert isolated and enzymatically cleaved by the selective restriction endonuclease, are ligated (joined) through the action of ligase enzyme to the vector DNA. As a result, an rDNA molecule known as cloning-vector-insert DNA construct is produced. Direct Ligation of Cohesive Termini (DNA Ligase) The target gene can be inserted
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