March 14, 2023

Following three culture media are commonly used to perform the tests for sterility: Nutrient Broth : This medium is used for aerobic microorganisms and have the following features: The value of redox potential (Eh) of this medium is too high to allow the growth of anaerobes. The culture medium, such as soyabean casein digest broth, Hartley’s

For the evaluation of efficiency, potency, and reactivity of disinfectants following techniques are used: Tube dilution and agar plate method, Cup-plate, filter paper, and cylinder plate method, Ditch-plate or giant colony method, Kelsey-Sykes test, In-use dilution test, Phenol coefficient test – Rideal-Walker test, and Chick-Martin test. Tube Dilution and Agar Plate Method The chemical disinfecting