March 18, 2023

This method relies on the diffusion of an antibiotic from a vertical cavity or a cylinder 2 through the solidified agar layer in a petri plate. The growth of test microorganisms is observed to be inhibited in a circular area or zone around the cavity containing antibiotic solution. The steps involved in cup-plate method are

This method depends on the microbial culture growth in a uniform solution of antibiotic in a fluid medium favourable to its rapid growth in the absence of antibiotic. This method involves a shorter incubation period (4-5 hours) for the growth of test microorganism. However, this assay method is affected by the presence of solvent residues

Antibiotics are the agents that kill or reduce the bacterial growth. They belong to a class of antimicrobials (a larger group also comprising of anti-viral, anti-fungal, and anti- . parasitic drugs). Antibiotics are chemicals either derived from or produced by the microorganisms (i.e., bugs or germs such as bacteria and fungi). The methods of microbiological