September 5, 2023

The desired antibodies should be extracted from a media sample of cultured hybridomas or a sample of ascites fluid. The contaminants in the cell culture sample consists of growth factors, hormones, and transferrins. The in vivo sample however, contains host antibodies, proteases, nucleases, nucleic acids, and viruses. Other secretions by the hybridomas (like cytokines) may

Production of MAbs in culture bottles is low (5−10μg/ml), thus to increase the yield the hybrid cells are grown as ascites in the peritoneal cavity of mice. The ascitic fluid contains 5−20mg of MAb/ml, which is much superior to the in vitro cultivation techniques. Collection of MAbs from ascitic fluid has a heavy risk of contamination by pathogenic